Laboratory of Plant Physiology and Biochemistry

The main areas of work of the Laboratory are: study of physiological and biochemical strategies for the adaptation of species and varieties of cultivated plants; creation of new methods for assessing the resistance of plants (varieties, lines, etc.) to the action of adverse environmental factors; development of theoretical foundations for increasing plant resistance through the use of physiologically active substances; development and improvement of methodological approaches to the biochemical analysis of plants.

The Laboratory performs the following tasks:

• physiological and biochemical support and support of breeding programs of the Institute;
• development and implementation of methodological approaches for the study of physiological and biochemical strategies for the adaptation of cereals, legumes and oilseeds to abiotic stress factors (hypo- and hyperthermia, drought, salinity, etc.);
• creation of methodological approaches to increase plant resistance to abiotic stressors by using new environmentally friendly resistance inductors (plant hormones and their analogues, signaling mediators, donors of gasotransmitters, etc.);
• development and improvement of methods for assessing the state of the antioxidant system of plants;
• assessment of the content of osmolytes and indicators of the water regime of plants;
• determination of fatty acid composition of oil;
• screening of the stress-protective effect of new synthetic biologically active compounds.

 

      

Offers for scientific cooperation and provision of services

The laboratory is equipped with modern laboratory equipment for conducting experiments aimed at studying the mechanisms of plant resistance to adverse factors and evaluating the biological activity of synthetic compounds, including using spectrophotometric, electrophoretic and chromatographic methods.

It is possible to conclude agreements with research institutions for joint research. Cooperation with agricultural enterprises of various forms of ownership is possible on a commercial basis.

2025

Kolupaev Y.E., Kokorev A.I., Kobyzeva L.N., Sakhino T.V., Barabolia O., Yastreb T.O. Priming with no donor sodium nitroprusside to activate germination and reduce oxidative damage in aged wheat and triticale seeds. Agriculture and Forestry, 2025. 71 (1): 07-26. https://doi:10.17707/AgricultForest.71.1.01 (Q3)

Taraban D.A., Karpets Yu.V., Yastreb T.O., Lugova A.A., Pysarenko V.M., Kolupaev Yu.E. Effect of melatonin priming on seed germination, carbohydrate metabolism, and antioxidant system in Triticum aestivum (Poaceae) under drought and salt stress. Ukrainian Botanical Journal, 2025, 82(2): 128–143. [In Ukrainian]. https://doi.org/10.15407/ukrbotj82.02.128 (Q3)

Yastreb T.O., Shkliarevskyi M.A., Kolupaev Yu.E. Quantitative determination of amylase activity in germinating cereal grains using agar plates and ImageJ software. Botanica, 2025. 31(2): 54–63. https://doi.org/10.35513/Botlit.2025.2.1 (Q3)

Shakhov I.V., Relina, L.I., Pyshchalenko M.A., Kolupaev Y.E. Activation of wheat seed germination under drought and salt stresses by γ-aminobutyric acid priming: Relationship with changes in ROS generation and osmolyte content. Notulae Scientia Biologicae, 2025. 17(2), 12366. https://doi.org/10.55779/nsb17212366 (Q3)

Chmielowska-Bak J., Kolupaev Y.E., Blume Y.B. Editorial: Plant stress – a threat to food security. Front. Plant Sci. 2025. 16, 1631524. https://doi.org/10.3389/fpls.2025.1631524 (Q1)

Kolupaev Yu.E., Shevchenko M.V., Shkliarevskyi M.A., Dmitriev A.P. Cellular Mechanisms of Inducing Plant Resistance to Stressors by β-Aminobutyric Acid. Cytology and Genetics, 2025, 59(4): 369–387. https://doi.org/10.3103/S009545272504005X (Q3)

Kolupaev Yu.E., Yastreb T.O., Yemets A., Blume Y. Nitric oxide functional relationships with nitrogen-containing stress metabolites: Role in plant adaptation to adverse abiotic factors. Nitric Oxide, 2025, 159: 126-146. https://doi.org/10.1016/j.niox.2025.10.003 (Q2)

Shakhov I.V., Yastreb T.O., Sakhno T.V., Kolupaev Yu.E. Involvement of Nitric Oxide in γ-Aminobutyric Acid-Induced Cellular Mechanisms of Wheat Seedling Adaptation to Water Deficit. Cytology and Genetics, 2025, 59(6): 580–595. https://doi.org/10.3103/S0095452725060106 (Q3)

Kolupaev Yu.E., Shkliarevskyi M.A. Accumulation of proline in vegetative organs of Triticum aestivum (Poaceae) under drought conditions in the early phases of development: A meta-analysis of data. Ukrainian Botanical Journal, 2025. 82(4): 277–290. [In Ukrainian]. https://doi.org/10.15407/ukrbotj82.04.277 (Q3)

Yastreb, T.O., Shevchenko, M.V., Kobyzeva, L.N., Oboznyi, A.I., Kolupaev, Y.E. β-Aminobutyric Acid Promotes Germination of Aged Triticale Seeds and Alleviates Oxidative Stress. Phyton-International Journal of Experimental Botany, 2025. 94(10): 3125–3143. https://doi.org/10.32604/phyton.2025.071822 (Q3)

Shakhov I.V., Yastreb T.O., Taraban D.A., Oboznyi A.I., Shkliarevskyi M.A., Ryabchun N.I., Kolupaev Yu.E.Activation of triticale grain germination under high temperature and simulated drought by nitric oxide donor and its relationship with carbohydrate metabolism and resistance to oxidative stress. Botanica, 2025, 31(4): 155–175. https://doi.org/10.35513/Botlit.2025.4.2 (Q3)

Kolupaev Yu.E., Relina L.I., Oboznyi A.I., Ryabchun N.I., Vasko N.I., Kolomatska V.P., Leonov O.Yu. Stress metabolites in wheat: role in adaptation to drought. Ukr. Biochem. J. 2025, 97(3): 13-41. https://doi.org/10.15407/ubj97.03.013 (Q4)

Yastreb T.O., Kosová K., Vítámvás P., Cit Z., Shakhov I.V., Kolupaev Y.E. Dehydrin content and status of antioxidant and osmoprotective systems of etiolated Triticum aestivum seedlings of different genotypes under osmotic stress. Journal of Crop Health. 2025. 77, 115 https://doi.org/10.1007/s10343-025-01190-0 (Q2)

2024

Kolupaev Yu.E., Taraban D.A., Kokorev A.I., Yastreb T.O., Pysarenko V.M., Sherstiuk E., Karpets Yu.V. Effect of melatonin and hydropriming on germination of aged triticale and rye seeds. Botanica, 2024, 30(1): 1–13. https://doi.org/10.35513/Botlit.2024.1.1 (Q4)

Kolupaev, Yu. E., Kokorev, O. I., Shevchenko, M. V., Marenych, M. M., & Kolomatska, V. P. Participation of γ-aminobutyric acid in cell signaling processes and plant adaptation to abiotic stressors. Studia Biologica, 2024, 18(1): 125–154. [in Ukrainian]. doi:10.30970/sbi.1801.752 (Q4)

Kolupaev Y.E., Shkliarevskyi M.A., Pyshchalenko M.A., Dmitriev A.P. 2024: Nitric oxide: functional interaction with phytohormones and applications in crop production. Agriculture and Forestry, 70(1): 379-411. https://doi.org/10.17707/AgricultForest.70.1.24 (Q3)

Kolupaev Y.E., Taraban D.A., Karpets Y.V., Kokorev A.I.,· Yastreb T.O., Blume Y.B.,·Yemets A.I. Involvement of ROS and calcium ions in developing heat resistance and inducing antioxidant system of wheat seedlings under melatonin’s effects. Protoplasma, 2024, 261(5):975–989. https://doi.org/10.1007/s00709-024-01952-z (Q1)

Kolupaev, Y.E., Shakhov, I.V., Kokorev, A.I., Dyachenko A.I., Dmitriev A.P. The Role of Reactive Oxygen Species and Calcium Ions in Implementing the Stress-Protective Effect of γ-Aminobutyric Acid on Wheat Seedlings Under Heat Stress Conditions. Cytol. Genet., 2024, 58(2), 81–91 https://doi.org/10.3103/S0095452724020063 (Q4)

Shakhov I.V., Kokorev A.I., Yastreb T.O., Dmitriev A.P., Kolupaev Yu.E. Increasing germination and antioxidant activity of aged wheat and triticale grains by priming with gamma-aminobutyric acid. Ukrainian Botanical Journal, 2024, 81(4): 290–304. [in Ukrainian]. https://doi.org/10.15407/ukrbotj81.04.290 (Q3)

Kolupaev, Y.E., Shakhov I.V., Kokorev A.I., Relina L.I., Dyachenko A.I., Dmitriev A.P. Gamma-aminobutyric acid induction of triticale protective systems under drought, salt stress or a combination of the two. Turkish Journal of Botany, 2024, 48(5): 235-248. https://doi.org/10.55730/1300-008X.2812 (Scopus, Q2)

Kolupaev Y.E., Ryabchun N.I., Relina L.I., Yastreb T.O., Yegorova N.Y., Kolomatska V.P. Exogenous nitric oxide and hydrogen sulfide as biotechnological tools for enhancing plant adaptation to cold. Notulae Scientia Biologicae, 2024, 16(3), 12064. https://doi.org/10.55779/nsb16312064 (Q4)

Yastreb T.O., Kokorev A.I., Dyachenko A.I., Shevchenko M.V., Marenych M.M., Kolupaev Yu.E. Indices of carbohydrate metabolism and antioxidant system state during germination of aged wheat and triticale seeds treated with H2S donor. Ukr. Biochem. J. 2024, 96(5): 79-95. https://doi.org/10.15407/ubj96.05.079 (Q4)

Akhunov A.A., Nurmatova M.I., Khashimova N.R., Buriev Z.T., Abdurakhmanov I.Y., Kolupaev Y.E. (2024): Responses of antioxidant system of various cotton genotypes to heat stress at the juvenile stage of ontogenesis. Agriculture and Forestry, 70 (1): 7-26 https://doi.org/10.17707/AgricultForest.70.1.01 (Q3)

Kolupaev Yu.E., Ryabchun N.I., Leonov O.Yu., Kokorev A.I., Taraban D.A., Shakhov I.V., Shkliarevskyi M.A., Yastreb T.O., 2024: Functioning of the antioxidant and osmoprotective systems of Triticum aestivum cultivars growing under soil drought conditions. Botanica, 30(3): 102–116. https://doi.org/10.35513/Botlit.2024.3.1 (Q3)

Kolupaev Y.E., Yemets A., Yastreb T.O., Blume Y. Functional interaction of melatonin with gasotransmitters and ROS in plant adaptation to abiotic stresses. Front. Plant Sci., 2024, 15:1505874. doi: 10.3389/fpls.2024.1505874 (Q1)

Kolupaev Y.E., Yastreb T.O., Dmitriev A.P.Gasotransmittersas Key Members of the Signaling Network Regulating Stomatal Response: Interaction with Other Molecules. Phyton-International Journal of Experimental Botany, 2024. 93, (12): 3151-3195/ https://doi.org/10.32604/phyton.2024.057922 (Q3)

Kolupaev Y.E., Yastreb T.O., Shkliarevskyi M.A. Dmitriev A.P. 2024: Salicylic Acid and Its Interaction with Other Signal Mediators and Plant Hormones in Shaping Adaptive Responses of Plants to Abiotic Stress. In: Yastreb T.O., Kolupaev Y.E., Yemets A.I., Blume Y.B. (Eds.) Regulation of Adaptive Responses in Plants. N.Y.: Nova Science Publishers, pp. 111-160. https://doi.org/10.52305/TXQB2084

Yastreb T.O., Kolupaev Y.E., Dmitriev A.P. 2024: Role of Jasmonate and Jasmonate Signaling Components in Plant Adaptation to Salt Stress. In: Yastreb T.O., Kolupaev Y.E., Yemets A.I., Blume Y.B. (Eds.) Regulation of Adaptive Responses in Plants. N.Y.: Nova Science Publishers, pp. 161-207. https://doi.org/10.52305/TXQB2084

Kolupaev Y.E., Taraban D.A., Karpets Y.V., Beschasnyi S.P., Kravets O.A., Relina L.I.,Yemets A.I., Blume Y.B. 2024: Melatonin as an Emerging New Phytohormone and Its Role in Plant Adaptation to Abiotic Stress Factors. In: Yastreb T.O., Kolupaev Y.E., Yemets A.I., Blume Y.B. (Eds.) Regulation of Adaptive Responses in Plants. N.Y.: Nova Science Publishers, pp. 209-257. https://doi.org/10.52305/TXQB2084

2023

Kolupaev, Y.E., Yemets, A.I., Yastreb, T.O., Blume, Y.B. The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants. Frontiers in Plant Science, 2023, 14, 1128439. https://doi.org/10.3389/fpls.2023.1128439 (Q1)

Kolupaev, Y.E., Taraban, D.A., Karpets, Y.V., Makaova B.E., Ryabchun N.I., Dyachenko, A.I., Dmitriev, O.P. Induction of Cell Protective Reactions of Triticum aestivum and Secale cereale to the Effect of High Temperatures by Melatonin. Cytology and Genetics, 2023, 57(2), pp. 117–127. https://doi.org/10.3103/S0095452723020068 (Q4)

Kolupaev, Y.E.; Yastreb, T.O.; Dmitriev, A.P. Signal Mediators in the Implementation of Jasmonic Acid’s Protective Effect on Plants under Abiotic Stresses. Plants 2023, 12, 2631. https://doi.org/10.3390/plants12142631 (Q1)

Kolupaev Yu.E., Shakhov I.V., Kokorev A.I., Kryvoruchko L., Yastreb T.O. Gamma-aminobutyric acid modulates antioxidant and osmoprotective systems in seedlings of Triticum aestivum cultivars differing in drought tolerance. Ukr. Biochem. J., 2023, 95(5) pp. 85-97. https://doi.org/10.15407/ubj95.05.085 (Q4)

Karpets, Y.V., Taraban, D.A., Kokorev, A.I., Yastreb, T.O., Kobyzeva, L.N., Kolupaev, Y.E.  Response of wheat seedlings with different drought tolerance to melatonin action under osmotic stress. Agriculture and Forestry, 2023, 69 (4), pp. 53-69. https://doi.org/10.17707/AgricultForest.69.4.05 (Q3)

Kolupaev, Y.E., Yastreb, T.O., Ryabchun, N.I.,Yemets, A.I., Dmitriev, O.P., Blume, Y.B. Cellular Mechanisms of the Formation of Plant Adaptive Responses to High Temperatures. Cytology and Geneticst, 2023, 57(1), pp. 55–75. https://doi.org/10.3103/S0095452723010048 (Q4)

Kolupaev, Y.E., Yastreb, T.O., Ryabchun, N.I.,Kokorev A.I., Kolomatska, V.P., Dmitriev, A.P. Redox homeostasis of cereals during acclimation to drought. Theoretical and Experimental Plant Physiology. 2023. 35. рр.133-168. https://doi.org/10.1007/s40626-023-00271-7 (Q2)

Yastreb, T.O., Kolupaev, Y.E., Kokorev, A.I., Ryabchun, N.I., Маkaova B.E., Zmiievska, O.A., Pospielova, G.D. Indices of antioxidant and osmoprotective systems in seedlings of winter wheat cultivars with different frost resistance. Ukrainian Biochemical Journal, 2023, 95(1), pp. 73–84. https://doi.org/10.15407/ubj95.01.073 (Q4)

Kolupaev, Y.E., Yastreb, T.O., Ryabchun, N.I., Kuzmyshyna, N.V., Marenych, M.M., Ryabchun, V.K. Signaling and protection systems in the adaptation of plants to cold. Journal of Central European Agriculturet, 2023, 24(1), pp. 202–215. https://doi.org/10.5513/JCEA01/24.1.3776 (Q3)

Kolupaev, Y.E., Маkaova, B.E., Yastreb, T.O., Ryabchun N.I., Tyshchenko V.M., Barabolia, O.V., Shkliarevskyi, M.A. Growth responses of wheat seedlings of different varieties to heat-stress and their relation to the antioxidant system state and osmolytes accumulation. Biologicni Studii, 2023, 17(1), pp. 81–97. https://doi.org/10.30970/sbi.1701.707 (SQ4)

Kolupaev, Yu. E.. Yastreb, T. O., Ryabchun, N. I., Kuzmyshyna, N.V., Shkliarevskyi, M. A., Barabolia, O., & Pysarenko, V. M. Response of Triticum aestivum seedlings of different ecological and geographical origin to heat and drought: relationship with resistance to oxidative stress and osmolyte accumulation. Agriculture and Forestry, 2023. 69(2):83-99. https://doi.org/10.17707/AgricultForest.69.2.07 (Q3)

Yastreb T.O., Kokorev A.I., Makaova B.E., Ryabchun N.I., Sakhno T.V., Dmitriev A.P., Kolupaev Yu.E. Response of the antioxidant system of wheat seedlings with different genotypes to exogenous prooxidants: the relationship with resistance to abiotic stressors. Ukrainian Biochemical Journal, 2023, 95(6), pp. 81-96. https://doi.org/10.15407/ubj95.06.081 (Q4)

e-mail: plant_biology@ukr.net